Ham¡¯s F12K,IC-2036-AGS

(+L-Glutamine)

BACKGOUND 

Ham¡¯s F12K was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

FEATURES
Theoretical pH : 7.3 ¡À 0.3

Osmolality : 297 mOsm/kg ¡À 10 %  

Colour : salmon - pink, clear solution

Sterility tests :

-Bacteria in aerobic and anaerobic conditions

-Fungi and yeasts

Endotoxin : <1 EU/ml
Size
500ML , 500MLx10.
Cell growth test

Medium tested for the ability to support CHO-K1 or Hela cell growth.

Composition

Displayed on web site; also available on request

Recommended use

-Respect storage conditions of the product

-Do not use the product after its expiry date

-Store product in an area protected from light (not necessary for saline solutions).

-Manipulate the product in aseptic conditions (e.g. : under laminar air flow)

-Wear clothes adapted to the manipulation of the product to avoid contamination (e.g. : gloves, mask, hygiene cap, overall¡­)

The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or veterinary applications.

Usage

Supplements, such as antibiotics, should be added as sterile supplements to the medium. Storage conditions and shelf-life of supplemented product will be affected by the nature of the supplements. Sterile serum should not be refiltered before or after being added to sterile medium because growth promoting capacity may be reduced upon re-filtration.

STORAGE

Store at 2-8¡æ For 12 Months.