DMEM Medium (Low Glucose), IC-2031-S
Product Introduction
Dulbecco''s Modified Eagle Medium (DMEM) was developed based on MEM (Minimum Essential Medium). Compared to MEM, DMEM contains a 2-fold increase in amino acids and a 4-fold increase in vitamins, along with the addition of non-essential amino acids, trace iron ions, and sodium pyruvate. DMEM was initially designed with a glucose concentration of 1000 mg/L (low-glucose type), and a formulation with 4500 mg/L glucose (high-glucose type) was later developed. It is now widely used for the culture of various cell types. DMEM low-glucose medium is more suitable for the culture of slower-metabolizing, adherent-dependent cells.
This product contains amino acids, vitamins, inorganic salts, and other components necessary for various cell culture types, but does not contain proteins, lipids, or growth factors. Therefore, this product must be supplemented with serum or serum-free additives before use.
Product Features
1. Low Glucose Formulation: Contains 1000 mg/L D-glucose, suitable for slower-metabolizing and adherent-dependent cells.
2. Complete Nutrient Profile: Includes 4 mM L-glutamine, 1 mM sodium pyruvate, and 3700 mg/L NaHCO₃.
3. Stringent Quality Control: Tested negative for bacteria, fungi, and mycoplasma; endotoxin levels <1 EU/mL.
4. Sterile-Filtered: 0.1 µm filtered to ensure sterility.
5. Broad Application: Suitable for a wide range of adherent cell types.
Specifications
Size: 500 mL
Product Form: Liquid
Concentration: 1¡Á
pH: 7.2-7.4
D-Glucose: 1000 mg/L
L-Glutamine: 4 mM
Sodium Pyruvate: 1 mM
NaHCO₃: 3700 mg/L
Phenol Red: 15 mg/L
HEPES: -
Endotoxin: <1 EU/mL
Sterility: Negative for bacteria, fungi, and mycoplasma
Storage and Stability
Storage Conditions: Store at 2-8¡æ, protected from light. Do not freeze.
Shipping Conditions: Ambient temperature
Shelf Life: 18 months from date of manufacture. After opening, use within 4-6 weeks when stored properly and handled aseptically.
Protocol (For Reference Only)
Important: Use aseptic technique when handling medium. Warm to 37¡æ before use.
1. Preparation of Complete Medium: Supplement DMEM Low Glucose with fetal bovine serum to a final concentration of 5-10% and add antibiotics as required. Mix gently.
2. Medium Pre-warming: Warm the required volume to 37¡æ for 15-30 minutes before use.
3. Cell Feeding: Aspirate spent medium, replace with fresh pre-warmed complete medium, and return cells to 37¡æ, 5% CO₂ incubator.
4. Subculturing: When cells reach 80-90% confluency, detach with trypsin-EDTA, neutralize with complete medium, and passage as appropriate.
5. Monitoring: Observe phenol red color indicator daily to assess pH.
Precautions
1. For research use only. Not for diagnostic or therapeutic use.
2. Handle aseptically to avoid contamination.
3. Do not freeze-thaw. Store at 2-8¡æ protected from light.
4. Supplemented complete medium should be used within 2-4 weeks.
5. This formulation does not contain HEPES; ensure adequate CO₂ atmosphere (5%).
FAQ (Simplified)
Q1: What cell types are suitable for DMEM Low Glucose?
A1: DMEM Low Glucose is suitable for slower-metabolizing, adherent-dependent cells, primary cells, and certain stem cell cultures.
Q2: What is the difference between DMEM low glucose and high glucose?
A2: Low-glucose DMEM contains 1000 mg/L D-glucose, while high-glucose DMEM contains 4500 mg/L. Low glucose is preferred for slower-growing and primary cells.
Q3: Does this medium contain L-glutamine?
A3: Yes. This formulation contains 4 mM L-glutamine.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-2031-S
Product Name: DMEM Medium (Low Glucose)
Size: 500 mL
Suggested Retail Price: CNY ¥95.00 / USD $9.50 / EUR €11.40 / JPY ¥1710.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
