DMEM/F12(1:1) Mediun (IC-2029-S)
Product Introduction
This product is a sterile, ready-to-use 1:1 mixture of Dulbecco''s Modified Eagle Medium (DMEM) and Ham''s F-12 Nutrient Mixture. DMEM/F12 combines the high concentrations of nutrients, vitamins, and amino acids from DMEM with the rich variety of additional components from Ham''s F-12, producing a highly enriched basal medium that supports the growth of a wide spectrum of mammalian cell types. This formulation is particularly well-suited for culturing primary cells, stem cells, and fastidious cell lines that require a rich nutritional environment. The medium contains 3151 mg/L D-glucose, 2.5 mM L-glutamine, 0.5 mM sodium pyruvate, and 2438 mg/L sodium bicarbonate, and is supplemented with phenol red as a pH indicator. It is formulated without HEPES, relying on the sodium bicarbonate/CO₂ buffer system for pH regulation under standard 5% CO₂ incubation conditions. The medium is manufactured under stringent quality control conditions with endotoxin levels <1 EU/mL and is tested negative for bacteria, fungi, and mycoplasma.
Product Features
1. Rich Nutrient Formulation: 1:1 combination of DMEM and Ham''s F-12 provides a comprehensive nutritional environment for diverse cell types including primary cells and stem cells.
2. Optimized Glucose Concentration: Contains 3151 mg/L D-glucose, providing adequate energy substrate without the excessive glycolytic drive of high-glucose formulations.
3. Complete Supplementation: Includes L-glutamine (2.5 mM), sodium pyruvate (0.5 mM), and sodium bicarbonate (2438 mg/L) for convenient ready-to-use culture.
4. Stringent Quality Control: Tested negative for bacteria, fungi, and mycoplasma contamination; endotoxin levels certified <1 EU/mL.
5. Sterile-Filtered: 0.1 µm filtered to ensure sterility for sensitive cell culture applications.
Specifications
Size: 500 mL；Product Form: Liquid；Concentration: 1×；pH: 7.2-7.4
D-Glucose: 3151 mg/L；L-Glutamine: 2.5 mM；Sodium Pyruvate: 0.5 mM；NaHCO₃: 2438 mg/L
Phenol Red: 8.1 mg/L；HEPES: -；Endotoxin: <1 EU/mL
Sterility: Negative for bacteria, fungi, and mycoplasma
Storage and Stability
Storage Conditions: Store at 2-8°C, protected from light. Do not freeze.
Shipping Conditions: Ambient temperature
Shelf Life: The product is stable for 18 months from the date of manufacture when stored as directed. After opening, use within 4-6 weeks when stored properly and handled aseptically.
Protocol (For Reference Only)
Important: Use aseptic technique when handling medium to prevent contamination. Warm medium to 37°C before use for optimal cell growth. This product has been sterilized by 0.1 µm filtration; maintain sterile technique during use.
1. Preparation of Complete Medium: Supplement DMEM/F12 Medium with fetal bovine serum to a final concentration of 5-10% and add antibiotics (e.g., penicillin-streptomycin) as required for specific cell lines. Mix gently by swirling.
2. Medium Pre-warming: Place the required volume of complete medium in a 37°C water bath or incubator for 15-30 minutes before use to minimize thermal shock to cells.
3. Cell Feeding: Aspirate spent medium from the culture vessel, replace with fresh pre-warmed complete DMEM/F12 Medium, and return cells to the incubator maintained at 37°C with 5% CO₂.
4. Subculturing: When cells reach 80-90% confluency, detach adherent cells using trypsin-EDTA, neutralize with complete medium, centrifuge if required, and resuspend in fresh complete DMEM/F12 Medium for seeding into new vessels.
5. Monitoring: Observe phenol red color indicator daily to assess pH changes; medium should appear pink-red at physiological pH and turn yellow-orange as acidity increases from cellular metabolism.
Precautions
1. This product is for research use only and is not intended for diagnostic or therapeutic use.
2. This product has been sterilized by 0.1 µm filtration; handle aseptically to avoid contamination.
3. To maintain optimal performance, do not freeze-thaw this product. Store at 2-8°C protected from light.
4. This formulation does not contain HEPES; ensure adequate CO₂ atmosphere (5%) for proper pH maintenance during culture.
5. Supplemented complete medium should be used within 2-4 weeks when stored at 4°C as glutamine degrades over time.
FAQ (Simplified)
Q1: What cell types are typically cultured in DMEM/F12?
A1: DMEM/F12 is widely used for culturing primary cells, stem cells (including mesenchymal stem cells and neural stem cells), prostate epithelial cells, mammary epithelial cells, and various fastidious cell lines. It is also commonly used as a basal medium for serum-free and low-serum culture systems.
Q2: What is the difference between DMEM/F12 and standard DMEM?
A2: DMEM/F12 is a 1:1 mixture that combines DMEM''s high nutrient concentrations with Ham''s F-12''s broader range of trace elements, vitamins, and amino acids. It provides a richer and more comprehensive nutritional environment than DMEM alone, making it suitable for cell types that require more complex media formulations.
Q3: Does this medium contain phenol red?
A3: Yes. This formulation contains 8.1 mg/L phenol red as a pH indicator. If phenol red is undesirable for specific applications (e.g., estrogen-sensitive assays), a phenol red-free formulation should be used.
Q4: How long is the shelf life after opening?
A4: After opening, the medium should be used within 4-6 weeks when stored at 2-8°C and handled aseptically. Aliquot the medium into smaller volumes for daily use to minimize the risk of contamination.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of culture conditions including serum concentration and supplementation is recommended for specific cell lines.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data resulting from product use or storage.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
|
Cat./REF. |
Size |
Price($） |
Price(�) |
Price(￥/CNY） |
Price(￥/JYP） |
|
IC-2029-S |
500ml |
$20.00 |
� 24.00 |
￥200.00 |
￥3,900.00 |
