Recombinant Human Interleukin-6(IL-6)
Cat.No.:CYTH0003
Human Interleukin-6
Interleukin-6 (IL-6) is an interleukin that in humans is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti- inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. Furthermore, It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The human IL-6 is a single non-glycosylated polypeptide chain containing 183 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6R¦Áchain (CD126), and the signal- transducing component gp130 (also called CD130).
Source: Escherichia coli.
Molecular Weight:Approximately 20.8 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids.
Size:5¦Ìg/20¦Ìg/1000¦Ìg
AA Sequence:VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM
Purity:>96% by SDS-PAGE and HPLC analyses.
Biological Activity:Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0x107 IU/mg.
Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8 ng/ml, corresponding toa specific activity of >1.25x106 IU/mg.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 ¦Ìm filtered concentrated solution in PBS, pH 7.4.
Endotoxin:Less than 1.0 EU/¦Ìg of rHuIL-6 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Ü -20¡æ. Further dilutions should be made in appropriate buffered solutions.
Shipping:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
l-12 months from date of receipt, -20¡æ to -70¡æ as supplied.
l-1 month, 2¡æ to 8¡æ under sterile conditions after reconstitution.
l-3 months, -20 to -70¡æ under sterile conditions after reconstitution.
Usage:This material is offered by InCellGene for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
