Recombinant Human TGF¦Â1/TGFbeta1

Cat.No.:

CYT-P01137

Product Background

Human Transforming Growth Factor - beta 1

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some nonredundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. TGF beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF beta 1 shares 100% aa identity with pig, dog and cow TGF beta 1, and 99 % aa identity with mouse, rat and horse TGFbeta 1.

Product Information

Source

Chinese Hamster Ovary cell line, CHO

Molecular Weight

Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 12 kDa under reducing conditions, a disulfide-linked homodimer of two 112 amino acid glycosylated polypeptide chains.

AA Sequence

Ala279-Ser390; Accession # P01137

Biological Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/mL. The specific activity of rHuTGF-¦Â1 is approximately 2.5 ¡Á 104U/¦Ìg, which is calibrated against human TGF-¦Â1 Standard.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 0.2 ¦Ìm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA.

Endotoxin

Less than 0.1 EU/¦Ìg of rHuGDF- 15/MIC- 1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ¡Ü -20 ¡ãC. Further dilutions should be made in
appropriately buffered solutions.

Purity

> 97 % by SDS-PAGE analyses.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 ¡ãC as supplied.

1 month, 2 to 8 ¡ãC under sterile conditions after reconstitution.

3 months, -20 to -70 ¡ãC under sterile conditions after reconstitution.

Usage Information

This  product  is  offered  by  BioCytoSci only  for Scientific Research & Laboratory  USE. NOT FOR OTHER USE.

Order Information

Cata.No.

Quantity

Price($)

Price($)

Availability

CYT-P01137

5ug

$185.00

€203.50

In stock

CYT-P01137

100ug

$980.00

€1,078.00

In stock

CYT-P01137

500ug

$4,200.00

€4,620.00

In stock

Free Delivery on orders over $200.00.

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