RPMI Medium 1640(IC-2025-S)

Description

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Contents

This RPMI Medium 1640 is modified as follows:

With L-glutamine,HEPES,Phenol Red

Without Sodium Pyruvate

The complete formulation is available.

Using RPMI 1640 Medium

RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12 and PABA, which are not found in Eagle''''''''''''''''''''''''''''''''s Minimal Essential Medium or Dulbecco''''''''''''''''''''''''''''''''s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with L-glutamine minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5¨C10% CO₂ environment to maintain physiological pH.

Product use

For human ex vivo tissue and cell culture processing applications.

CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using BioCytoSci® RPMI 1640 medium in a manufacturing process, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).

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