NCI-H1975(ICGE-NCI-H1975,IC-82485)
Catalog No.
Size
Price
IC-82485
10^6
$467.00
IC-82485
10^6
€513.70
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | lung |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma; non-small cell lung cancer |
| Gender | female |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
|
|
|
|---|---|
| Derivation |
The line was established in July 1988. The tissue donor was a non-smoker.
|
| Clinical Data |
female
The tissue donor was a non-smoker.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: 1:3 to 1:6
Medium Renewal: two to three times weekly
|
| Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
|
