Recombinant Human TGF-β1/TGF-beta 1, CYT-P01137L
Product Introduction
Transforming Growth Factor β-1 (TGF-β1) is a secreted protein belonging to the TGF-β family. TGF-β1 is abundantly expressed in bone, articular cartilage, and chondrocytes, and is increased in osteoarthritis (OA). TGF-β1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGF-β1 peptide. TGF-β1 may also form heterodimers with other TGF-β family members. It has been found that TGF-β1 is frequently upregulated in tumor cells. Mutations in this gene result in Camurati-Engelmann disease.
Product Features
1. Mammalian Expression System: Produced in a mammalian expression system for proper folding and post-translational processing.
2. High Purity: Greater than 95% as determined by reducing SDS-PAGE.
3. High Biological Activity: ED50 of 4-40 pg/mL as measured by inhibition of IL-4-dependent proliferation of TF-1 human erythroleukemic cells (QC verified).
4. Lot-to-Lot Consistency: Three independent lots tested for activity and plotted on the same graph to demonstrate consistency.
5. Low Endotoxin: <0.01 EU/µg as determined by LAL test.
Specifications
Size: 10 µg / 50 µg / 100 µg
Protein Accession: P01137
Alternative Names: Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB
Molecular Weight: 12.8 kDa
Apparent Molecular Weight: 13 kDa, reducing conditions
Expression Region: Ala279-Ser390
Formulation: Lyophilized from a 0.2 µm filtered solution of 50 mM Glycine-HCl, 150 mM NaCl, 4% Mannitol, pH 2.5
Endotoxin: <0.01 EU/µg (LAL test)
Purity: Greater than 95% by reducing SDS-PAGE
Biological Activity: ED50 of 4-40 pg/mL, measured by inhibition of IL-4-dependent proliferation of TF-1 human erythroleukemic cells
Storage and Stability
Shipping Conditions: Shipped at ambient temperature. Upon receipt, store immediately at the temperature listed below.
Storage Conditions:
- Lyophilized protein: Store at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution: Store at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples: Stable at ≤ -20°C for 3 months.
Protocol (For Reference Only)
Important: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/mL.
1. Reconstitution: Dissolve the lyophilized protein in distilled water. Allow to dissolve completely with gentle mixing; do not vortex.
2. Aliquoting: Aliquot the reconstituted solution into single-use volumes to minimize freeze-thaw cycles.
3. Storage: Store aliquots at ≤ -20°C for up to 3 months. Reconstituted solution may be stored at 2-8°C for 2-7 days.
4. Activity Assay: Biological activity is measured by inhibition of IL-4-dependent proliferation of TF-1 human erythroleukemic cells, with an ED50 of 4-40 pg/mL.
5. Working Concentration: Optimal working concentrations should be determined empirically for each cell type and experimental system.
Precautions
1. Always centrifuge tubes before opening.
2. Do not mix by vortex or pipetting; gentle inversion or swirling is recommended.
3. It is not recommended to reconstitute to a concentration less than 100 µg/mL to avoid protein loss.
4. Aliquot reconstituted solution to minimize freeze-thaw cycles.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: How should I reconstitute this protein?
A1: Dissolve the lyophilized protein in distilled water to a concentration of at least 100 µg/mL. Do not vortex or pipette vigorously.
Q2: What is the biological activity of this protein?
A2: The ED50 is 4-40 pg/mL as measured by inhibition of IL-4-dependent proliferation of TF-1 human erythroleukemic cells.
Q3: How should I store the reconstituted protein?
A3: Reconstituted solution can be stored at 2-8°C for 2-7 days. For longer storage, aliquot and store at ≤ -20°C for up to 3 months. Avoid repeated freeze-thaw cycles.
Q4: What is the endotoxin level?
A4: <0.01 EU/µg as determined by LAL test.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of working concentrations is recommended for specific cell types and experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CYT-P01137
Product Name: Recombinant Human TGF-β1/TGF-beta 1
Size: 10 µg / 50 µg / 100 µg
Suggested Retail Price:
10 µg: CNY ¥2960.00 / USD $296.00 / EUR �355.20 / JPY ¥53280.00
50 µg: CNY ¥6500.00 / USD $650.00 / EUR �780.00 / JPY ¥117000.00
100 µg: CNY ¥9600.00 / USD $960.00 / EUR �1152.00 / JPY ¥172800.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
