Tris Acetate-EDTA Buffer (TAE Buffer, 50X), IC-5302

Product Introduction

TAE (Tris-Acetate-EDTA) buffer is a commonly used electrophoresis buffer for DNA gel electrophoresis. The 1¡Á working solution contains 40 mM Tris-acetate and 1 mM EDTA, pH 8.0. TAE is suitable for the separation of DNA fragments in agarose gels, particularly for fragments larger than 1 kb and for applications requiring DNA recovery for downstream enzymatic reactions. Compared to TBE buffer, TAE has lower buffering capacity but provides better DNA recovery and is preferred for preparative gels and applications where DNA will be purified for cloning or sequencing. This product is supplied as a 50¡Á concentrated stock solution and should be diluted 50-fold with distilled water before use.

Product Features

1. Standard DNA Electrophoresis Buffer: Widely used for agarose gel electrophoresis of DNA.

2. 50¡Á Concentrated Format: Convenient storage and cost-effective; dilute 50-fold before use.

3. Good DNA Recovery: Preferred for preparative gels and DNA fragment purification.

4. Suitable for Large DNA Fragments: Effective separation of DNA fragments larger than 1 kb.

Specifications

Size: 500 mL

Concentration: 50¡Á

1¡Á Working Solution Composition: 40 mM Tris-acetate, 1 mM EDTA, pH 8.0

Application: DNA agarose gel electrophoresis

Storage and Stability

Storage Conditions: Store at room temperature (15-25¡ãC). If precipitation occurs at low temperatures, warm to room temperature and mix before use.

Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed.

Protocol (For Reference Only)

Important: Ensure any precipitate is fully dissolved before dilution. Use distilled or deionized water for dilution.

1. Preparation of 1¡Á Working Solution: Dilute the 50¡Á TAE buffer 1:50 with distilled water (e.g., 20 mL of 50¡Á TAE plus 980 mL of distilled water to prepare 1 L of 1¡Á TAE). Mix thoroughly.

2. Agarose Gel Preparation: Dissolve agarose in 1¡Á TAE buffer by heating. Cool and cast the gel.

3. Electrophoresis: Submerge the gel in 1¡Á TAE buffer. Load DNA samples and run at appropriate voltage.

4. Buffer Reuse: 1¡Á TAE buffer may be reused for multiple gel runs, but prolonged reuse may reduce resolution. Replace buffer when bands become diffuse or resolution decreases.

Precautions

1. If precipitation occurs during storage, warm to room temperature and mix until fully dissolved.

2. Do not use the 50¡Á concentrate directly for electrophoresis; always dilute to 1¡Á.

3. TAE has lower buffering capacity than TBE; for extended or high-voltage electrophoresis, replace buffer or recirculate if necessary.

4. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: How do I prepare 1¡Á TAE from the 50¡Á concentrate?

A1: Dilute the 50¡Á TAE 1:50 with distilled water (e.g., 20 mL of 50¡Á TAE plus 980 mL of water for 1 L of 1¡Á TAE).

Q2: What is the difference between TAE and TBE?

A2: TAE has lower buffering capacity than TBE but provides better DNA recovery from gels, making it preferred for preparative applications. TBE offers higher buffering capacity and is better for extended electrophoresis.

Q3: Can 1¡Á TAE be reused?

A3: Yes. 1¡Á TAE may be reused several times, but replace it when resolution decreases or bands become diffuse.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.

3. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: IC-5302

Product Name: Tris Acetate-EDTA Buffer (TAE Buffer, 50X)

Size: 500 mL

Suggested Retail Price: CNY ¥275.00 / USD $27.50 / EUR €33.00 / JPY ¥4950.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.