0.2 µm PVDF Blotting Membrane, IC-8220
Product Introduction
This product is a polyvinylidene fluoride (PVDF) blotting membrane with a pore size of 0.2 µm, designed for protein transfer and immunodetection in Western blotting applications. PVDF membranes offer high protein binding capacity, mechanical strength, and chemical resistance, making them a preferred choice for Western blotting, protein sequencing, and protein analysis. The 0.2 µm pore size is recommended for proteins with molecular weights below 20 kDa, as smaller pores prevent protein loss through the membrane during transfer, ensuring efficient binding of low molecular weight proteins and peptides. The membrane is supplied in convenient roll format, allowing users to cut membranes to the desired size. PVDF membranes must be pre-wetted with methanol before use to ensure proper hydration and protein binding.
Product Features
1. High Protein Binding Capacity: PVDF surface provides strong protein binding for sensitive detection.
2. Small Pore Size: 0.2 µm pore size is optimized for proteins below 20 kDa, preventing protein loss during transfer.
3. Mechanical Strength: Durable membrane suitable for stripping and reprobing.
4. Chemical Resistance: Compatible with organic solvents, acids, and bases.
5. Convenient Roll Format: Allows flexible sizing for different gel dimensions.
Specifications
Size: 26.5 cm ¡Á 2 m / roll; 26.5 cm ¡Á 4 m / roll
Pore Size: 0.2 µm
Material: Polyvinylidene fluoride (PVDF)
Application: Western blotting, protein transfer, immunodetection
Storage and Stability
Storage Conditions: Store at room temperature in a dry environment, protected from dust and direct sunlight.
Shelf Life: The product is stable for 36 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: PVDF membrane must be pre-wetted with 100% methanol before use. Do not allow the membrane to dry out during transfer and detection.
1. Membrane Activation: Immerse the PVDF membrane in 100% methanol for 15-30 seconds until uniformly translucent. Rinse in deionized water, then equilibrate in transfer buffer for at least 5 minutes.
2. Transfer Assembly: Assemble the transfer sandwich according to standard protocols, placing the activated membrane against the gel.
3. Protein Transfer: Perform electrophoretic transfer according to standard protocols.
4. Post-Transfer Processing: Proceed directly to blocking and immunodetection. Ponceau S staining may be performed to assess transfer efficiency.
5. Stripping and Reprobing: The membrane can be stripped and reprobed multiple times.
Precautions
1. Always pre-wet with methanol before use; failure to do so results in poor protein binding.
2. Do not allow the membrane to dry out during transfer and detection.
3. Wear gloves and use clean forceps to handle the membrane.
4. Cut membranes to size before activation.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What protein sizes are suitable for 0.2 µm PVDF membrane?
A1: The 0.2 µm pore size is recommended for proteins below 20 kDa. For proteins above 20 kDa, a 0.45 µm membrane is generally sufficient.
Q2: Why is methanol activation necessary?
A2: PVDF is naturally hydrophobic. Methanol activation hydrates the membrane, enabling effective protein binding.
Q3: Can this membrane be stripped and reprobed?
A3: Yes. PVDF membranes can be stripped and reprobed multiple times due to their mechanical strength and chemical resistance.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-8220
Product Name: 0.2 µm PVDF Blotting Membrane
Size: 26.5 cm ¡Á 2 m / roll; 26.5 cm ¡Á 4 m / roll
Suggested Retail Price:
26.5 cm ¡Á 2 m / roll: CNY ¥2350.00 / USD $235.00 / EUR €282.00 / JPY ¥42300.00
26.5 cm ¡Á 4 m / roll: CNY ¥3450.00 / USD $345.00 / EUR €414.00 / JPY ¥62100.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
