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Recombinant Human EGFR (Epidermal Growth Factor Receptor) |
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Cat.No.: |
CYT-P00533 |
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Product Background |
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Recombinant Human EGFR |
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The EGFR subfamily of receptor tyrosine kinases is composed of EGFR, ErbB2, ErbB3 and ErbB4. The EGFR shares 43% - 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2, resulting in cell signaling, heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, epigen, and so on. Its signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target. |
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Product Information |
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Source |
Mammalian Cells |
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Molecular Weight |
69.6 KDa(90-120 KDa, reducing conditions) |
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AA Sequence |
Recombinant Human Epidermal Growth Factor Receptor is produced by our Mammalian expression system and the target gene encoding Leu25-Ser645 is expressed with a 6His tag at the C-terminus. |
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Biological Activity |
Loaded Anti-Human EGFR mAb-Fc on Pro A Biosensor, can bind Recombinant Human EGFR-His with an affinity constant of 7.23 nM as determined in BLI assay. |
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Physical Appearance |
Lyophilized from a 0.2 ¦Ìm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4. |
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Formulation |
Lyophilized from a 0.2 ¦Ìm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4. |
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Endotoxin |
< 0.01 EU/µg as determined by LAL test. |
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Reconstitution |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
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Purity |
Greater than 95% as determined by reducing SDS-PAGE. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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12 months from date of receipt, -20 to -70 ¡ãC as supplied. |
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1 month, 2 to 8 ¡ãC under sterile conditions after reconstitution. |
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3 months, -20 to -70 ¡ãC under sterile conditions after reconstitution. |
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Usage Information |
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This product is offered by BioCytoSci only for Scientific Research & Laboratory USE. NOT FOR OTHER USE. |
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Order Information |
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Cata.No. |
Quantity |
Price($) |
Price($) |
Price(£¤) |
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CYT-P00533 |
10ug |
$156.00 |
€171.60 |
£¤1,560.00 |
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CYT-P00533 |
50ug |
$460.00 |
€506.00 |
£¤4,600.00 |
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CYT-P00533 |
1mg |
$4,200.00 |
€4,620.00 |
£¤42,000.00 |
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Free Delivery on orders over $200.00. |
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We Devoted Ourselves To The Development Of Biomedical Research Reagent. |
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