Recombinant Mouse IL-4,CYT-P07750

Description

Mouse Interleukin-4(IL-4) is a monomeric, Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four ¦Á­helix structure. IL­4 exerts its effects through two receptor complexes, Participates in at least several B-cell activation processes as well as of other cell types. IL­4 is primarily expressed by Th2­biased CD4+T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL­4 plays a dominant role in the development of allergic inflammation and asthma. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Expression Sequence:Recombinant Mouse Interleukin-4 is produced by our E.coli expression system andthe target gene encoding His23-Ser140 is expressed.

Endotoxin: < 1 EU/µg as determined by LAL test.

Formulation:Lyophilized from a 0.2um filtered solution of 20mM PB,5% Sucrose,4% Mannitol, 0.1% PS-80,pH 6.5.
Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100¦Ìg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage

Lyophilized protein should be stored at ¡Ü -20¡æ, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8¡æ for 2-7 days.Aliquots of reconstituted samples are stable at ¡Ü -20¡æ for 3 months.

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