CAMK2¦Á Kinase Assay Kit,IC-6582
Background
CAMK2¦Áis a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family.CAMK2¦Áis abundant in the brain as a major constituent of the postsynaptic density and is required for hippocampal long-term potentiation(LTP)and spatial learning.In addition to its Ca2+/calmodulin-dependent activity,CAMK2¦Ácan undergo autophosphorylation,resulting in Ca2+/calmodulin-independent activity.The protein level of CAMK2¦Áfluctuates during neuronal activity in cultured rat pup hippocampal neurons.The levels of CAMK2¦Áincreased with heightened neuronal activity(2).
1.Silva,A J.et al:Impaired spatial learning in alpha-calciumcalmodulin kinase II mutant mice.Science 257:206-211,1992.
2.Thiagarajan,T C.et al:Alpha-and beta-CaMKII:inverseregulation by neuronal activity and opposing effects on synaptic strength.Neuron 36:1103-1114,2002.
Usage
1.Dilute enzyme, substrate, ATP and inhibitors in Kinase Buffer.
2.Add to the wells of 384 low volume plate:
3.1 ¦̀l of inhibitor or (5% DMSO)
4.2 ¦̀lof enzyme (defined from table 1)
5.2 ¦̀lof substrate/ATP mix
6.Incubate at room temperature for 60 minutes.
7.Add 5 ¦̀l of ADP-Glo™ Reagent
8.Incubate at room temperature for 40 minutes.
9.Add 10 ¦̀l of Kinase Detection Reagent
10.Incubate at room temperature for 30 minutes.
11.Record luminescence (Integration time 0.5-1second).
Note:CAMK2¦Á Kinase Buffer: 40mM Tris,pH 7.5; 20mM MgCl2 ; 0.1mg/ml BSA; 50¦̀M DTT and Ca2+/Calmodulin solution (0.03¦̀g/¦̀l Calmodulin, 1mM Tris,pH 7.3 ,0.5mM CaCl2).
Storage
Store at -20¡ăC.
Order Information
|
Cat./REF. |
Size |
Price($£© |
Price(€) |
Price(£¤/CNY£© |
Price(£¤/JYP£© |
|
IC-6582 |
1000Tests |
$1,182.00 |
€ 1,418.40 |
£¤11,820.00 |
£¤235,218.00 |
