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CellPro Serum-free Medium (SF1801-02)
Click£º6684     Release date£º2015-10-11    Author£ºDavid-Pat     Source£ºOriginal

CellProTM Serum-free Medium(SF1801-02)

£¨Note:This Product has no any biology Visicles and completely artificial synthetic medium £©

Datasheet

Introduction

CellProTM Serum-free Medium (cat. no. SF1801-02) is a serum-free medium designed for the cultivation of a wide variety of mammalian cell types. The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of CellProTM Serum-free Medium  is approximately 3 mg/ml. CellProTM Serum-free Medium does not contain L-glutamine.

CellProTM Serum-free Medium  has been used to grow a variety of cells of primary origin and established cell lines. The medium may be used to support the fusion of cells during the formation of hybridomas.CellProTM Serum-free Medium has been used to grow cells of lymphoid origin including monocyte and macrophage cell lines. Epithelial and fibroblastic cells have been cultivated in CellProTM Serum-free Medium  and endothelial cells have been cultivated under reduced serum concentration (5% fetal bovine serum using CellProTM Serum-free Medium  as a base formulation).

CellProTM Serum-free Medium  has also been used as a complete medium to support the generation of viral particles for use in vaccine production.

Instructions for Use

CellProTM Serum-free Medium is a complete all-purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines. The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution per 500 ml.

Turbidity may develop CellProTM Serum-free Medium . Experiments have determined that the turbidity will not alter the performance of the product.

CellProTM Serum-free Medium should be stored at 2¡ãC - 8¡ãC. For cells in serum-containing medium weaning is NOT required.

Product Use Statement

THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures.

Ordering Information

Catalog Description number
SF1801-02  CellProTM Serum-free Medium

Size 500 ml without  L-glutamine

Partial List of Cells Cultivated with CellProTM Serum-free Medium :

Cell line

Source

Cell type

HEL, N-10

Human

Fetal lung diploid fibroblast

HeLa

Human

Uterine cancer

HuL-1,2

Human

Liver (normal)

HuK-1

Human

Kidney (normal)

HuS-1AT

Human

Skin

HEC

Human

Embryonic cancer

HL-60

Human

Acute promyelocytic leukemia

Raji

Human

Burkitt¡¯s lymphoma

EB-3

Human

Burkitt¡¯s lymphoma

K-562

Human

Chronic myelocytic leukemia

HNK

Human

Neonatal kidney (primary)

HTC29

Human

Colon Cancer

TT

Human

Medulary thyroid tumor

MB231

Human

Breast carcinoma

U138

Human

Glioma

FM3A

Mouse

Breast cancer

NS-1

Mouse

Myeloma

L

Mouse

Subcutaneous

P388D1

Mouse

Macrophage-like

P815

Mouse

Mast cell tumor

T3

Mouse

Pituitary

B82

Mouse

L cells ¨Cconnective tissue

RPL-1

Rat

Peritoneum

RSP-2

Rat

Spleen

RLG-1

Rat

Lung

Lym-1

Rat

Lymph node

RCR-1

Rat

Brain

235-1, MNQ

Rat

Pituitary

GC, GH3

Rat

Pituitary

CA77

Rat

Medulary thyroid tumor

Rat-1

Rat

Fibroblast

JTC-12

Monkey

Kidney

COS1, COS7

African green monkey

SV40 transformed kidney


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