Bacterial Scavenger,CP-CS-0411

Product Description
Bacterial contamination is the most common contamination in cell culture, which mainly comes from laboratory environment, laboratory personnel, lab clothes, lab consumables, shared reagents, etc. Even the addition of double antibodies during cell culture often causes contamination due to careless operation. Bacterial contaminated culture media usually appear turbid, with a decrease in pH and yellowing. In the early stages of contamination, there is no significant change observed with the naked eye. Only by carefully observing under an inverted microscope can bacteria "pass through" the culture medium. It is recommended to observe carefully every day to detect bacterial contamination in a timely manner. Generally, bacterial contamination progresses quickly, and changes can be significantly observed with the naked eye after about one or two days of contamination. Under the microscope, black spots can be observed swimming in the culture medium, cells become round or collapse, and fall off from the wall and die.
Bacterial scavengers are suitable for removing bacterial contamination during cell culture processes. They can significantly inhibit bacterial growth in just 12 hours and remove bacterial contamination in about a week, maximizing the rescue of precious cells and reducing losses caused by bacterial contamination. This product has been tested on hundreds of cells in a cell bank and verified through long-term experiments, and can completely eliminate bacterial contamination during the cell culture process.

Usage method
Take out the bacterial scavenger from the -20 ¡æ refrigerator, centrifuge the reagent tube instantaneously (3000rpm, 3-5 seconds), and place it on the EP tube rack. Spray the surface of the reagent tube with 75% alcohol and perform aseptic operation in the biosafety cabinet. Operating procedures for removing bacterial contamination using T25 cell culture bottles as an example
For cells with occasional black spots passing through the culture medium but no obvious turbidity, which are in the early stage of bacterial contamination, it is necessary to immediately replace them with a complete culture medium containing a bacterial cleaning agent. The recommended dilution ratio is 2000 times, for example, adding 3 ¦Ì L of bacterial cleaning agent to 6mL of complete culture medium and mixing it well. Continuous drug addition and cultivation for 1-2 weeks can effectively remove bacterial contamination.

For cells with sudden yellowing and obvious desertification and turbidity of the culture medium, bacterial contamination is already very serious. It is necessary to increase the drug concentration as appropriate to improve the efficiency of removing bacteria. It is recommended to increase the concentration to 1000 x for removal, such as adding 6 ¦Ì L of bacterial cleaning agent to 6mL of complete culture medium and mixing well. Change the liquid twice a day. It is recommended that you enter the laboratory in the morning to change the medium with one dose. In the afternoon, you should change the medium with one dose before leaving the laboratory. Repeat this process the next day and the third day. You can change the liquid once a day from the the fourth day. After continuous dosing and culture for 1 to 2 weeks (or three times of passage), sterility test will determine whether there is still bacterial pollution.

Note:
1.Before changing the medium or passaging the adherent cells, discard the old culture medium and gently wash the cell surface twice with PBS.
2.If the cells reach the passable ratio, please passage them in a timely manner and place them in a new cell culture bottle. The process of replacing the bottle can also effectively avoid contamination by residual bacteria on the wall of the culture bottle;
3. After the bacteria are completely removed, they must be passaged and placed in new cell culture bottles. After adding medication to the new bottles for 1-2 days, the bacterial cleaning agent can be removed to avoid secondary contamination caused by residual bacteria on the walls of the old bottles after removal;
4. Due to the fragility of embryonic stem cells (H1, H9, iPS), if contamination is severe, it is recommended to use a concentration of 2000 x to remove bacteria.
Operating procedures for preventing bacterial contamination
If cells need to be continuously cultured and there are shared experimental instruments, consumables, reagents, or large-scale bacterial contamination in the laboratory, it is recommended to take regular preventive measures every 2-3 weeks. Add an appropriate amount of bacterial scavenger to the cell culture medium, with a recommended dilution ratio of 3000 times. For example, mix 6mL of cell culture medium with 2 ¦Ì L of bacterial scavenger. Continuous drug addition and cultivation for one week can effectively prevent bacterial contamination or inhibit bacterial proliferation.

Product advantages
1. High efficiency, can effectively inhibit bacterial proliferation within 12 hours;
2. High specificity, specifically clearing bacterial contamination;
3. High broad-spectrum, highly effective against both Gram positive and Gram negative bacteria, and also has activity against multidrug-resistant bacteria;
4. No drug resistance, the active ingredients are mainly peptides and will not develop drug resistance;
5. High safety, almost non-toxic to cells, has been validated on hundreds of cell types.
Transportation and storage methods
Ice pack transportation- Store at 20 ¡æ away from light, with a shelf life of 2 years.
Special Reminder
1. Please read the instructions carefully before using this reagent;
2. This product is sterilized by 0.1 ¦Ì m filtration, and does not require filtration when using it. It can be directly added to the culture medium for use;
3. This reagent has patented technology that does not freeze when stored at -20 ¡æ, and does not require thawing when used. It can be taken out from -20 ¡æ and used immediately;
4. In order to achieve the best therapeutic effect, it is recommended to prepare and use the drug containing culture medium immediately. If the drug containing culture medium is not used up, it should be stored in a refrigerator at 4 ¡æ in the dark and used up within 2 weeks. Before using the culture medium, it should be preheated to 37 ¡æ;
5. If individual cells are sensitive to this reagent and their growth rate is significantly affected, it is recommended to reduce the use or conduct dilution testing;
6. After treatment with bacterial cleaning agents, there will be good prevention and removal effects. However, if there are still pollution sources in the environment, consumables, and reagents, cells may be contaminated again, so appropriate preventive measures need to be taken;
7. When adding this product for bacterial prevention and elimination, there is no need to add dual antibodies (penicillin streptomycin);
For your safety and health, please wear lab coats and disposable gloves when operating;
9. This product is for research or further production use only and should not be used for diagnosis or treatment.

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