Hemin /Panhematin,16009-13-5

Cat.No.:IC-035319

Product Information

Biological Activity

Hemin chloride, a substrate of heme oxygenase (HO)-1, induces HO-1 expression on a variety of cells to exert anti-oxidant and anti-inflammatory roles.
A dramatic reduction of cell-associated M. hyorhinis DNA in a dose-dependent manner, with > 90% inhibition at 100 ¦Ìm Hemin chloride. Hemin chloride treatment profoundly inhibited intracellular M. hyorhinis DNA levels within 10 h to nearly undetectable levels after 48 h[1]. Macrophage exposed to Hemin chloride exhibits modulation of non-opsonic phagocytosis of aged RBCs, ability to kill bacteria and secretion of cytokines.Translocation and sequestration of CD36 within the intracellular storage in the Hemin chloride treated macrophages. It in-turn modulates the global cytokine secretion from macrophages. CD36 has strong affinity for Hemin chloride with a dissociation constant of 1.26 ¡À0.24 ¦ÌM[3]. Hemin chloride treatment decreased cell proliferation to 62.5 %, 51.3 %, and 38.8 % in PA-TU-8902, BxPC-3 and MiaPaCa-2 cancer cells, respectively. Enhancement of anti-proliferative effects of statins by Hemin chloride, documented as decreased cell proliferation after 48 h of co-treatment[5]. The presence of Hemin chloride in irradiated lung cancer cells enhanced the productivity of initial ROS, resulting in lipid peroxidation and subsequent ferroptosis[6].
In HDM-induced asthmatic mouse model, Hemin chloride-DCEVs inhalation reduced eosinophils infiltration and mucus secretion in the airway, decreased the levels of IL-4, IL-5, and IL-13 in the lung and the number of Th2 cells in mediastinal lymph nodes (MLNs), and increased the number of Treg cells in MLNs[2]. Hemin chloride-preconditioned mice exhibited preserved renal cell function, and the tubular injury score at 72h indicated that tubular damage was prevented[4]. When compared with wild-type littermates, the mortality for SS Cttn+/- mice trended to be lower after Hemin chloride infusion and these mice exhibited less severe lung injury and less necroptotic cell death[7].

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species

Mouse

Rat

Rabbit

Guinea pig

Hamster

Dog

Weight (kg)

0.02

0.15

1.8

0.4

0.08

10

Body Surface Area (m2)

0.007

0.025

0.15

0.05

0.02

0.5

Km factor

3

6

12

8

5

20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by  (Animal B Km / Animal A Km)
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Order Information

Quantity

Price($)

Price(€)

Availability

500mg

$60.00

€72.00

In stock

5g

$420.00

€504.00

In stock

50g

$2,940.00

€3,528.00

In stock

Free Delivery on orders over $350.00.

Chemical Information

Molecular Weight

652

Formula

C34H32ClFeN4O4

CAS Number

16009-13-5

Purity

>98.00%

Solubility

2.5 mg/mL in NH4OH,1M NaOH : 6.67 mg/mL.

Storage

4¡ãC, protect from light, stored under nitrogen

Reference

[1]. Huang H, Dabrazhynetskaya A, et,al.Hemin chloride activation abrogates Mycoplasma hyorhinis replication in chronically infected prostate cancer cells via heme oxygenase-1 induction. FEBS Open Bio. 2021 Oct;11(10):2727-2739. doi: 10.1002/2211-5463.13271. Epub 2021 Sep 9. PMID: 34375508; PMCID: PMC8487054.

[2]. Wu Y, Yu Q, et,al.Hemin chloride-primed dendritic cells suppress allergic airway inflammation through releasing extracellular vesicles. J Leukoc Biol. 2022 Apr;111(4):837-848. doi: 10.1002/JLB.3A0321-175R. Epub 2021 Jul 23. PMID: 34296788; PMCID: PMC9292814.

[3]. Banesh S, Layek S, et,al. Hemin chloride acts as CD36 ligand to activate down-stream signalling to disturb immune responses and cytokine secretion from macrophages. Immunol Lett. 2022 Mar;243:1-18. doi: 10.1016/j.imlet.2022.01.004. Epub 2022 Jan 31. PMID: 35104496.

[4]. Chen HH, Lu PJ, et,al. Heme oxygenase-1 ameliorates kidney ischemia-reperfusion injury in mice through extracellular signal-regulated kinase 1/2-enhanced tubular epithelium proliferation. Biochim Biophys Acta. 2015 Oct;1852(10 Pt A):2195-201. doi: 10.1016/j.bbadis.2015.07.018. Epub 2015 Jul 30. PMID: 26232688.

[5]. Vanova K, Boukalova S, et,al. Heme oxygenase is not involved in the anti-proliferative effects of statins on pancreatic cancer cells. BMC Cancer. 2016 May 12;16:309. doi: 10.1186/s12885-016-2343-9. PMID: 27175805; PMCID: PMC4866069.

[6]. Almahi WA, Yu KN, et,al. Hemin chloride enhances radiosensitivity of lung cancer cells through ferroptosis. Exp Cell Res. 2022 Jan 1;410(1):112946. doi: 10.1016/j.yexcr.2021.112946. Epub 2021 Nov 24. PMID: 34826424.

[7]. Jones NM, Sysol JR,et,al. Cortactin loss protects against Hemin chloride-induced acute lung injury in sickle cell disease. Am J Physiol Lung Cell Mol Physiol. 2022 Jun 1;322(6):L890-L897. doi: 10.1152/ajplung.00274.2021. Epub 2022 May 3. PMID: 35503995; PMCID: PMC9169831.

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