D-Luciferin (potassium salt),115144-35-9

Cat.No.:

IC-0195355

Product Information

Biological Activity

D-Luciferin potassium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects.D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly Photinus pyralis, the enzyme more frequently used in techniques of in vitro and optical imaging[1].Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in DPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 ¦Ìm filter by drawing through 5-10 mL of sterile H2O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 ¦Ìm syringe filter.Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection[2].Inject with 10 ¦ÌL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 ¦ÌL for a 20 g mouse for a standard 150 mg/kg injection.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species

Mouse

Rat

Rabbit

Guinea pig

Hamster

Dog

Weight (kg)

0.02

0.15

1.8

0.4

0.08

10

Body Surface Area (m2)

0.007

0.025

0.15

0.05

0.02

0.5

Km factor

3

6

12

8

5

20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by  (Animal B Km / Animal A Km)
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Order Information

Quantity

Price($)

Price(€)

Availability

100mg

$75.00

€90.00

In stock

200mg

$140.00

€168.00

In stock

500mg

$375.00

€450.00

In stock

Free Delivery on orders over $350.00.

Chemical Information

Molecular Weight

318.4

Formula

C11H7N2O3S2•K

CAS Number

115144-35-9

Purity

>98.00%

Solubility

10mg/mL in DMSO, 16.7mg/mL in DMF, 30mg/mL in Water

Storage

 -20¡æ

Reference

[1]. Giuseppe Meroni, et al. D-Luciferin, derivatives and analogues: synthesis and in vitro/in vivo luciferase-catalyzed bioluminescent activity. ARKIVOC 2009 (i) 265-288.

[2]. Inoue Y, et al. Timing of imaging after d-luciferin injection affects the longitudinal assessment of tumor growthusing in vivo bioluminescence imaging. Int J Biomed Imaging. 2010;2010:471408.

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