ExperPro WesternBlot Fast Blocking Buffer(1x)
Cat.No.:IC-8009
Description
ExperPro WesternBlot Fast Blocking Buffer contains a proprietary non-relevant protein for blocking excess binding sites in Western blotting applications. This blocking buffer has been optimized for use with precipitating substrates and yields a high signal-to-noise ratio in most applications. Additionally, this blocking buffer does not contain albumin or endogenous biotin and, therefore, may be used with avidin/streptavidin systems.Because no blocking reagent is optimal for all systems, empirical testing is essential to determine the appropriate blocking buffer for each Western blot system. Determining the proper blocking buffer can increase sensitivity and prevent nonspecific signal caused by cross-reactivity between the antibody and the blocking reagent.
Features
-Ready To Use: Direct use without the need to add any additional reagents or dilute.
-Efficiently Blocking: ExperPro WesternBlot Fast Blocking Buffer effectively solves the problem of long closure time, which usually only takes 5-15 minutes.
-Excellent Compatibility：ExperPro WesternBlot Fast Blocking Buffer is compatible with secondary antibodies that are compatible with horseradish peroxidase (HRP), alkaline phosphatase (AP), and biotin labeled (biotin). This product has added preservatives that do not affect the activity of HRP and AP, and will not interfere with the detection of HRP or AP labeled secondary antibodies. At the same time, this product does not contain biotin and will not interfere with biotin based detection.
Usage
1. Remove blot from the transfer unit.
2. Use a sufficient volume of blocking buffer to completely cover blot. Using large volumes of blocking and wash buffer often reduces nonspecific signal.
3. Incubate for approximately 5-15 minutes with agitation.
4.Remove the blocking buffer and continue Western blot procedure.
Storage
The product will be stable for 12 months under 2-8℃.
Order Information
Cat./REF.
Size
Price($）
Price(�)
Price(￥/CNY）
Price(￥/JYP）
IC-8009
100ml
$26.00
� 31.20
￥260.00
￥5,174.00
IC-8009
500ml
$65.00
� 78.00
￥650.00
￥12,935.00
Cat./REF.
Size
Price($）
Price(�)
Price(￥/CNY）
Price(￥/JYP）
IC-8009
100ml
$26.00
� 31.20
￥260.00
￥5,174.00
IC-8009
500ml
$65.00
� 78.00
￥650.00
￥12,935.00
Troubleshooting
|
Problem |
Possible cause |
Remedy |
|
No signal or weak signal |
Poor transfer efficiency |
Optimize the membrane transferring procedure |
|
Insufficient antigen |
Increase the amount of loaded antigen |
|
|
Make sure the blot have been store correctly to avoid the degradation of target protein |
||
|
The concentration of primary and secondary antibody is too low |
Increase the concentration of the primary and/or the secondary antibody |
|
|
Inappropriate storage/preparation of the ECL detection reagents |
Use HRP or HRP conjugates to check the applicability of ECL reagents |
|
|
Too short exposure time |
Extend exposure time |
|
|
Excessive signal |
Antigen or antibody excess |
Reduce the amount of loaded antigen |
|
Dilute the primary antibody and/or the secondary antibody |
||
|
Antigen or antibody excess |
Optimize the condition by reducing the amount of antigen, or the concentration of the primary antibody and/or secondary antibody. Initially, reduce the secondary antibody to 20% of the original usage |
|
|
Inappropriate blocking |
Try different blocking substrate such as gelatin, casein, skim milk or casein |
|
|
High Background |
Inadequate washing |
Increase the concentration of Tween-20 in washing solution |
|
Increase the washing steps between the hybridization procedures |
||
|
Extend washing time |
||
|
Overexposure to film |
Shorten the exposure time |
