ExperPro WesternBlot Fast Blocking Buffer(1x)

IC-8009

Background
Blocking Bufferg contains a proprietary non-relevant protein for blocking excess binding sites in  Western blotting applications. This blocking buffer has been optimized for use with precipitating substrates and yields a high signal-to-noise ratio in most applications. Additionally, this blocking buffer does not contain albumin or endogenous biotin and, therefore, may be used with avidin/streptavidin systems.Because no blocking reagent is optimal for all systems, empirical testing is essential to determine the appropriate blocking buffer for each Western blot system. Determining the proper blocking buffer can increase sensitivity and prevent nonspecific signal caused by cross-reactivity between the antibody and the blocking reagent. 
Usage Protocol
1.Example Procedure for Blocking Western Blots
2. Remove blot from the transfer unit.
3. Use a sufficient volume of blocking buffer to completely cover blot. Using large volumes of blocking and wash buffer often reduces nonspecific signal.
4. Incubate for approximately 10-15 minutes with agitation.
5. Remove the blocking buffer and continue Western blot procedure.

Storage

Store at 2-8 ℃.