Pyrrolidinedithiocarbamate ammonium,5108-96-3

Cat.No.:

IC-0124686

Product Information

Biological Activity

Pyrrolidinedithiocarbamate ammonium is a selective NF-¦ÊB inhibitor.
Pretreatment of cells with pyrrolidinedithiocarbamate (3-1000 ¦ÌM) dose-dependently attenuate IL-8 production. Furthermore, pyrrolidinedithiocarbamate (100 ¦ÌM) suppresses the accumulation of IL-8 mRNA. Pyrrolidinedithiocarbamate inhibits the activation of NF-¦ÊB, because pyrrolidinedithiocarbamate suppresses both NF-¦ÊB DNA binding and NF-¦ÊB-dependent transcriptional activity. NF-¦ÊB inhibition with pyrrolidinedithiocarbamate decrease IL-8 production by intestinal epithelial cells[1].
The DSS+pyrrolidinedithiocarbamate ammonium-treated groupII exhibits suppression of shortening of intestinal length and reduction of DAI score. Activated NF-¦ÊB level and IL-1¦Â and TNF-¦Á levels are significantly lower in DSS+pyrrolidinedithiocarbamate ammonium-treated groupII. These findings suggest that suppression of NF-¦ÊB activity by pyrrolidinedithiocarbamate ammonium can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1¦Â and TNF-¦Á), resulting in significant alleviation of colitis. pyrrolidinedithiocarbamate ammonium is useful for the treatment of ulcerative colitis[2].

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species

Mouse

Rat

Rabbit

Guinea pig

Hamster

Dog

Weight (kg)

0.02

0.15

1.8

0.4

0.08

10

Body Surface Area (m2)

0.007

0.025

0.15

0.05

0.02

0.5

Km factor

3

6

12

8

5

20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by  (Animal B Km / Animal A Km)
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Order Information

Quantity

Price($)

Price($)

Availability

100mg

$50.00

€55.00

In stock

500mg

$120.00

€132.00

In stock

1g

$180.00

€198.00

In stock

Free Delivery on orders over $350.00.

Chemical Information

Molecular Weight

164.28

Formula

C₅H₁₂N₂S₂

CAS Number

5108-96-3

Purity

>99.00%

Solubility

¡Ý 7.3mg/mL in DMSO, ¡Ý 12.975mg/mL in EtOH with gentle warming

Storage

4¡ãC, protect from light

Reference

[1]. N¨¦meth ZH, et al. Pyrrolidinedithiocarbamate inhibits NF-kappaB activation and IL-8 production in intestinal epithelial cells. Immunol Lett. 2003 Jan 2;85(1):41-6.
[2]. Qin JD, et al. Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-¦ÊB activation and CYP2E1 content of rats with immunological liver injury. Pharm Biol. 2014 Nov;52(11):1460-1466.

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